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Research protocols in animals have used doses ranging from 1 mcg/kg/day to 120 mcg/kg/day depending on the model and endpoint

[DOI] [PubMed] [Google Scholar] 4.Erotokritou-Mulligan I, Bassett EE, Bartlett C, et al

The preclinical literature is extensive and spans more than three decades, with animal model data generally supporting a manageable safety profile at the concentrations and study durations examined

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Increased cytoplasmic calcium levels in cardiomyocytes, arising from chronic overloading or mitochondrial dysfunction, are associated with altered protein folding, ER stress, and induction of FGF21 (113)